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The method comparison demonstrated better repeatability for OCT dimensions compared with histology. SD-OCTis possible in awake rabbits. Easy and reliable recognition of this VS may facilitate the positioning and make use of of rabbits as design species in real human macular and general retinal disease study.SD-OCT is possible in awake rabbits. Easy and reliable recognition regarding the VS may facilitate the positioning and use of rabbits as model species in human macular and general retinal illness anal