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Results In all bleached groups, H2O2 had infiltrated into the pulp chamber. The highest level of penetration was noted in group 2 (2.32 ± 0.25 µg), while the lowest level was noted in group 3 (1.85 ± 0.33 µg). The difference in this regard was significant between groups 2 and 3 (P = 0.024), but the differences between other groups were not statistically significant (P ≥ 0.42). Conclusion Considering the results of this study, it can be stated that hydroge peroxide penetration in to pulp chamber in diode laser activation of bleaching age