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ther study. The purpose of this study was to determine the effects of polymethylmetnacrylate (PMMA) spacer loaded with different concentrations of vancomycin on the proliferative, osteogenic, and angiogenic capacity of the induced membrane. Varying concentrations of vancomycin (0, 1, 2, 4, 6, 8, and 10g) were fully mixed with bone cement powder (40g), resulting in seven experimental groups. Hollow cylindrical PMMA spacers (10mm height, 3mm external diameter, and 0.8mm internal diameter) were formed by a mold and submerged in phosphate-b