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Customers and techniques NDM-5-producing E. coli isolate KY1497 was recognized into the urine sample of an individual hospitalized in a tertiary hospital in Japan. The complete genome sequence of isolate KY1497 was determined by short- and long-read sequencing with hybrid assembly, followed by multilocus sequence typing (MLST), core-genome phylogeny analysis, plasmid analysis, and transconjugation experiments. Results KY1497 had been classified as ST405 by MLST, and core-genome phylogeny exhibited the c