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05). The maximal isometric force/cross-sectional area generated by isolated membrane-permeabilised muscle fibres was determined. Results None of the 426 variants identified in 16 candidate genes were associated with MFM including 26 missense variants. Breed-specific differences existed in allele frequencies. Candidate gene differential expression and muscle fibre-specific force did not differ between MFM WB (143.1 ± 34.7 kPa) and non-MFM WB (140.2 ± 43.7 kPa) (P = .8). Main limitations RNA-seq-only assays transcripts expressed in skeletal