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6%) and Chitwan (6.6%). Coimbra mutation (592 CT) was found among 75.5% of the G6PD-deficient samples analysed and Mahidol (487 GA) and Mediterranean (563 CT) mutations were found in equal proportions in the remaining 24.5%. There was no specific geographic or ethnic distribution for the three mutations. This study has identified populations with moderate to high prevalence of G6PD deficiency which provides strong evidence supporting the WHO recommendations to screen G6PD deficiency at health facility level before