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To evaluate the bactericidal effect of silver nanoparticles and silver nanoparticles in combination with Nd-YAG laser against . A solution containing 100 μg of silver nanoparticles in 1 mL was prepared by adding 5 mL of 10 M solution of AgNO with 5 mL of 0.1 M sodium tricitrate. Synthesized silver nanoparticles were characterized using UV-visible spectrophotometer for optical studies and the transmission electron microscopic analysis for determining the size and shape of the nanoparticles. Groups are as follows group I-silver nanopar