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The gene was detected in 1/33 (3.03%) isolates from healthy ostriches, in 8/47 (17.05%) isolates from feces, and in 7/36 (19.44%) intestinal contents of diseased ostriches. The full-length sequences of 5 out of 15 -positive isolates from diseased ostriches showed 100% identity to each other as well as to the sequences available in GenBank. All of these five isolates produced NetB toxin . Type A is the most prevalent among isolates from ostriches in Vietnam. Especially, the study provides data emphasizing the role of NetB toxin in causin