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Model validation was carried out against averaged α factor gradient data from one plant, and against diurnal air flow measurements from another. The Benchmark Simulation Model 1 configuration was used to demonstrate the applicability of the proposed model - in estimation of blower energy consumption and peak air flow requirements - comparing it with constant and scheduled α factor-based approaches.Protein sensors based on allosteric enzymes responding to target binding with rapid changes in enzymatic activity are potential tools for homo