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Unlike adult fish, the larval fish showed significant genetic changes related to neurotoxicity. The hypothyroidism induced in the larval fish by the exposure might be potentially associated with the neurotoxic potential of EHMC. The implications of the observed hormonal and transcriptional-level changes in zebrafish at different life stages following long-term exposure warrant further investigation.Vacuum freeze-drying is a scientifically advanced method to prepare solid inoculants from oil degrading bacterium. The introduction of oil-d