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The aim of this study was to evaluate biomarkers of heat stress (HS) from an automatic milking system (AMS), the relationships between measurements of the temperature-humidity index (THI), reticulorumen pH and temperature, and some automatic milking systems parameters in dairy cows (rumination time (RT), milk traits, body weight (BW) and consumption of concentrate (CC)) during the summer period. Lithuanian Black and White dairy cows (n=365) were selected. The cows were milked with Lely Astronaut® A3 milking robots with free traffic. B