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The sidestreams produced during fish processing end in a separation tank where the resulting fractions follow biogas production or wastewater treatment. These streams can alternatively be used for production of protein-rich fungal biomass for e.g. fish feed applications, a product in increasing demand. These streams and upper streams originated during fish processing were used in this study for production of biomass using the edible filamentous fungus Rhizopus oryzae. The COD of the streams varied between 11 and 54 kg/m3 and, after fun