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Besides, current limitations and challenges of these materials, as well as their future prospects in the biomedical field will be discussed.Over the past 2 decades, zebrafish, Danio rerio, have become a mainstream laboratory animal model, yet zebrafish husbandry practices remain far from standardized. Feeding protocols play a critical role in the health, wellbeing, and productivity of zebrafish laboratories, yet they vary significantly between facilities. In this study, we compared our current feeding protocol for juvenile zebrafish (3