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This mutant showed PT resistance and exhibited no development problem or auxotrophy. The thiI gene had been more investigated for its usage as a selection marker in genome co-editing. Ribonucleoprotein complex comprising recombinant Cas9 nuclease and sgRNA targeting thiI or any other target gene (wA or sreA) ended up being ready and simultaneously introduced into A. oryzae RIB40. thiI and target gene double loss-of-function mutants were effectively chosen on PT-containing medium. thiI had been sh
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