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The emergence of this signal depends on the degree of disorder making both highly brittle fracture of low disorder materials, and ductile fracture of strongly disordered ones, unpredictable.Our aim was to assess whether airborne particle counting is an immediate indicator of biocontamination in controlled environment rooms with HEPA filters in a hospital. A prospective study was carried out in a tertiary care hospital between 2016 and 2018. The study was divided in two periods and the measurements were performed in different controlled