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guidelines to improve suction technology. This work also provides valuable insights to the improvement of several other extracellular recording technology in laboratory research or clinical settings. Using a multidisciplinary approach in neuroscience has tackled the regeneration enigma in the central nervous system for decades. Reported regeneration potential in mammals is lacking confirmation without a suitable paradigm. In vivo fetal experimentation offers an almost insurmountable drawback, though its feasibility has been shown long