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We found that chloroform frass extracts from P. xuthus larvae fed on Poncirus trifoliata leaves contained significant cytotoxic activity. Our findings demonstrate that screening of insect species using the 'TUATinsecta' database provides an important pipeline for discovering novel therapeutic agents that might be useful for mankind.The estimation of long-term groundwater recharge rate ([Formula see text]) is a pre-requisite for efficient management of groundwater resources, especially for arid and semi-arid regions. Precise estimation