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mellitus against GCA or whether it is only a mimic. Human milk oligosaccharides (HMOs) in breast milk contribute to the development of the neonatal microbiota and immune system. However, longitudinal studies examining HMO profiles of Chinese mothers remain scarce. We aimed to analyze HMO profiles, including their composition, concentrations, and changes during lactation, in milk of Chinese mothers. A total of 822 milk samples from 222 mothers were collected, of which 163 mothers provided single samples. Samples fro