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The part of Fe(II) from the humic acid (HA) transformation plus the aftereffects of humic acid Fe (HA-Fe) on simultaneous partial nitrification, anammox and denitrification (SNAD) system were examined. After adding Fe(II), the HA content decreased as well as the HA inhibition regarding the SNAD system was launched. Results showed that Fe(II) and HA formed the lower water-soluble HA-Fe, promoting the HA removal. HA-Fe with stronger electron transfer ability constituted the