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OCT resolved structural changes after SDF application as well as changes overtime. High resolution microscopy images confirm penetration of SDF into the samples. Such changes can potentially be monitored to determine if and when re-application of SDF is needed.A series of 2,6-diiminopyridine-derived macrocyclic ligands have been synthesized via [2+2] condensation around alkaline earth metal triflate salts. The inclusion of a tert-butyl group at the 4-position of the pyridine ring of the macrocyclic synthons results in macrocyclic compl