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Biofilms had been additionally examined by gasoline chromatography-mass spectrometry and confocal laser checking microscopy. All extracts with hot steeping showed higher inhibitory effects on biofilm development and cell viability and lower GTF levels compared to individuals with cool steeping (p less then 0.05). Hot steeping dramatically paid down microbial development (p less then 0.05) and maintained the pH. Catechins were only identified from hot steepin
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