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The understanding of U reprotoxicity requires the fine understanding of its speciation at molecular amount, i.e., its interacting with each other with cytosolic biomolecules. In this study, we concentrate on the U-protein interactions in gonads. A non-denaturating extraction protocol combined with size exclusion chromatography (SEC) permitted the separation of metal-protein complexes in ovaries of U-contaminated crazy roaches before their elemental recogniti