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The isolation of exoRNA requires the application of ultracentrifugation to separate cell-free RNA followed by detection utilizing real-time PCR, microarray, next-generation sequencing or Nano-String nCounter system. The employment of exoRNA enrichment panels have mainly increased the recognition sensitivity and specificity in comparison with old-fashioned diagnostic examinations. More over, using exoRNA as biomarkers can help early detection of chemo- and radio-resistance