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05) reduced the TNF-α expression and increased the epidermal thickness. Conclusion It can be concluded that the administration of eel (M. albus) extract therapy could help to reduce TNF-α expression and increase epidermal thickness in rat incision wounds. Copyright © Journal of Advanced Veterinary and Animal Research.Objective The present study aimed to assess for the first time, in Medina, the prevalence of Mycobacterium avium subsp. paratuberculosis in ruminants due to its potential zoonotic importance. Materials and methods A total of 823 sera samples an