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In the demyelinated corpus callosum, CD44 was localized on GFAP+ astrocytes and IBA1+ microglial cells. Despite a robust expression induction, Cd44-deficiency did not ameliorate cuprizone-induced pathology. Although further studies will be needed to examine the functional relevance of CD44 in the cuprizone model, the spatial and temporal expression pattern of CD44 will pave the way to evaluate its precise role in different (immune and non-immune) pathological conditions.Hundreds of millions of people worldwide are exposed to unacceptabl