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The trough serum concentration range of 10 to 20mg.L-1 was considered therapeutic. RESULTS All three patients had at least one infection by S. epidermidis, being one patient exhibit vancomycin-resistant S. epidermidis infection. All patients had stoppages in the vancomycin treatment, and the minimal inhibitory concentration was performed for only one patient. CONCLUSION This study showed the minimal inhibitory concentration clinical value in the treatment efficacy due to the necessity to reach a specific range of trough serum concentration