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Here, we evaluated the Deeplex Myc-TB targeted deep-sequencing assay for forecast of opposition to 13 anti-tuberculous drugs/drug courses, right applicable on sputum.With MTBC DNA tests, the restriction of detection had been 100-1000 genome copies for fixed resistance mutations. Deeplex Myc-TB captured in silico 97.1-99.3% of opposition phenotypes correctly predicted by WGS from 3651 MTBC genomes. On 429 isolates, the assay predicted 92.2% of 2369 first- and