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Protein sequencing was employed to verify the amino acid sequencing regarding the insulins; as a result, the standard had a 90% likelihood to personal insulin through the database, whereas the biosynthesized version had a 96% probability. The biological tasks of both variations for the SFC purified proteins had been assessed in vitro utilizing a MTT assay. The outcome indicated that the biological tasks of both samples were retained subsequent to SFC purification. This research effect