https://www.selleckchem.com/pr....oducts/sndx-5613.htm
Sperm DNA integrity has become one of the most discussed and promising biomarkers for the assessment of male fertility. However, an easy-to-apply method capable of estimating DNA fragmentation in the live fraction of spermatozoa has remained elusive, preventing this parameter from being fully applied in clinical settings. To validate a novel co-staining for the analysis of DNA fragmentation in membrane-intact spermatozoa. Normozoospermic semen samples were used to validate the co-staining consisting of acridine orange (AO) and LIVE/DE