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Our findings suggest the acute metabolic demands of singing are comparable with walking at a moderately brisk pace, hence, physical effects may contribute to the health and well-being benefits attributed to singing participation. However, if physical training benefits result remains uncertain. Further research including different singing styles, singers and physical performance impacts when used as a training modality is encouraged. ClinicalTrials.gov registry (NCT04121351). ClinicalTrials.gov registry (NCT04121351). Animal studies indicate a potential prot