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In addition, the improvement of 3D printing to use bioinks has also achieved significant progresses in fabricating living functional biofilms with specific structures. In the future, the combination of synthetic biology and techniques from other disciplines will lead to practical large-scale applications of biofilms.The high hydrophilicity of citicoline and its rapid metabolism are the two main obstacles hindering intact molecules from passing the blood-brain barrier. This study aimed to formulate citicoline-loaded niosomes (CTCNSMs) for efficient brain del