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NS1-specific Western blot analysis also detected anti-NS1 antibodies in sera of people who obtained several amounts of either among these two vaccines; however, many vaccinees whom received ≤3 doses were negative for NS1-specific antibodies. The share of NS1-specific antibodies to protection against TBE was demonstrated by immunization of mice with purified NS1 antigen, which led to a significant (p less then 0.01) prolongation associated with mean survival time after life-threatening v