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To test the hypothesis that telomere shortening and/or loss are risk factors for infertility. Retrospective analysis of the telomere status in patients with infertility using conventional cytogenetic data collected prospectively. Academic centers. Cytogenetic slides with cultured peripheral lymphocytes from 50 patients undergoing fertility treatment and 150 healthy donors, including 100 donors matched for age. Cytogenetic slides were used to detect chromosomal and telomere aberrations. Telomere length and telomere aberrations were analyzed