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Groups 2 and 3 showed greater agitation and aggression. Marked reduction in the level of aggressiveness and agitation was observed in group 4 as compared with all other groups evaluated during handling by the animal technician. Male hamsters housed in groups of 4 and females housed individually has reduced risks of accident during handling, thereby averting distress and consequent physiologic alterations. Avoiding these risks is essential to obtaining reliable experimental results. Eating disorders are widely recognized as serious illne