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01) in serum and PEDV-specific secretory IgA (SIgA) in saliva (P less then 0.01) and nasal secretions (P less then 0.01). An increased number of intestinal CD3+ T cells, IgA-secreting cells and intraepithelial lymphocytes (P less then 0.05) were also observed. Furthermore, the protein expression levels of interleukin-6 and interferon-γ, relative to the control PEDV infection, were also significantly elevated (P less then 0.05). The results of the present study indicate that nasal immunization is more effective at inducing the intestinal

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