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We report a study of cooperativity in the transition from a nonaqueous deep eutectic solvent (DES) to an aqueous solution. In some systems, a nonequilibrium region prevails at low water contents, while cooperativity is always observed at high water contents. Catechol-based mixtures exhibit a Hill constant (nH) of ∼ 3 and an overall ΔG° of ca. -3-5 kJ/mol for the transition from DES to aqueous solution. In contrast, o-phenylenediamine-based mixtures exhibit a shift from nH = 0 at low water contents to nH ∼ 12 at high water contents with
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