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Taken together, the current results indicate that flavopereirine exhibits good anticancer activity in HCC cells. Forkhead box O4 (FOXO4) has been demonstrated to be a tumor suppressor and proposed as target for treatment of a variety of cancer types. However, the role of FOXO4 in cholangiocarcinoma (CCA), a dangerous cancer of bile-duct epithelium, has rarely been explored. The proliferative rate of CCA cell lines KKU-213B, KKU-055 and KKK-D068 was investigated using the sulforhodamine B (SR assay. Levels of FOXO4, cyclin E1 (CCNE1), C

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