https://proteasesinhibitors.co....m/latest-perspective
The cell-wall active antibiotic vancomycin is often used to treat both methicillin-resistant (MRSA) and painful and sensitive (MSSA) attacks. Vancomycin intermediate S. aureus (VISA) variants can arise through de novo mutations. Here, we performed pilot experiments to build up a combined PCR/long-read sequencing-based method for detection of previously understood VISA-causing mutations. Primers were designed to produce 10 amplicons addressing 16 genetics linked to the
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