https://www.selleckchem.com/products/GDC-0449.html
Mice were challenged by the . The 726 bp of N-CfTX-1 were cloned in a vector of expression pET28a [+] and confirmed by PCR, sequencing and enzymatic analysis. Moreover, the recombinant protein was confirmed by SDS-PAGE and Western blotting. Then the antibody was isolated from mice serum and confirmed by ELISA test. The results showed that immunized mice tolerated 50x LD50 of jellyfish venom. The CfTX-1 recombinant protein was able to protect the BALB c mice against jellyfish venom. The produced protein can be used as a candidate for va
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