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Paratuberculosis or Johne's disease (JD) prevalence in Australia is low at the cow-level with varying herd-level prevalence. Control strategies incorporating vaccination are limited, suggesting opportunities for changes in regulatory oversight. In order to study this further, we examined the economic benefits of participation in JD control programmes in Australia with and without vaccination as well as knowledge, attitudes, and practices (KAP) relating to JD. We used an online questionnaire to gather information describing demographics