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Metal-organic frameworks (MOFs), with attractive properties such as high surface area, tunable porosity, designable functionality and excellent stability, have aroused tremendous interests of researchers as the matrices for enzyme immobilization. Recently, several efficient strategies including surface immobilization, post-synthetic infiltration and in situ encapsulation have been explored. The MOFs-immobilized enzymes, named enzymes@MOFs, show remarkably enhanced stability and recyclability, accelerating the cell-free biocatalysis in d