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It could be used routinely in a forensic frame to respond to judicial requests. Our method of age estimation is simpler and accurate. It could be used routinely in a forensic frame to respond to judicial requests. Demand for age determination by medical methods from legal authorities is of critical importance, especially for people in pubertal age. We planned this study to evaluate the potential utility of biochemical methods in these applications. We aim to investigate whether alkaline phosphatase (ALP), bone-specific alkaline phosphat