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Due to the fact that AA can be generated by the enzyme catalysis reaction between ALP and 2-phospho-l-ascorbic acid (AAP), the CDs-Ag+-OPD ratiometric system was applied to the determination of ALP successfully. The ratiometric fluorescence value of F454/F570 increases with increasing ALP concentration, with a linear range of 0.2 to 40 U/L and detection limit of 0.1 U/L. In addition, the CDs-Ag+-OPD ratiometric system was successfully applied to the detection of ALP in human serum samples.By utilizing the effective energy t

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