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This protocol defines methods for calculating female postmating habits, such as for example oviposition, feminine intimate receptivity, and mating plug ejection.During reproduction, male and female flies use wing vibration to create different acoustic signals. Males create a courtship tune before copulation that is easily acquiesced by unilateral wing vibration. In copula, females produce a definite sound design (copulation tune) with both wings. Sexual rejection of immature virgins and intense activities b

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