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OBJECTIVES/HYPOTHESIS Recent research has examined the nasal microbiome in rhinosinusitis and nondiseased states. Given immunologic alterations in allergic rhinitis (AR) and after allergen immunotherapy (IT), we evaluated the nasal microbiome in these conditions. STUDY DESIGN Cross-sectional comparison. METHODS In this cross-sectional study, nasal swabs for microbiome analysis were collected from three patient groups IT-naïve AR patients, AR patients undergoing IT for greater than 12 months, and a control group without sinonasal inflamma