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quantitative reverse transcription-polymerase chain reaction was performed to evaluate bone marker transcription. RESULTS The 3D model shows good cell viability and permits IL dosing. Additionally, three gene expression profiles are analysable. LIMITATIONS The model allows analysis of conventional markers; larger exploration is needed for better understanding osteoblast mechanobiology. However, it only allows an analysis over 3 days. CONCLUSION The results obtained by applying constant compressive forces to 3D osteoblastic cultures va