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Results verified that unknown isolates and subspecies included in the item could possibly be precisely identified by using this real time PCR method.Bacillus subtilis and Enterococcus faecium can be utilized probiotics. This study aimed to recognize the effect of live combined Bacillus subtilis R0179 and Enterococcus faecium R0026 (LCBE) on obesityassociated hyperlipidemia and instinct microbiota in C57BL/6 mice. Forty male C57BL/6 mice had been divided into four teams regular group (N team