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05 followed by different diagnostic tests confirmed the good fitting. The best values to acquire DF close to 1 were pH 3.5, methanol/ethyl acetate ratio 8713 and eluent volume 6 mL. The streamlined method was validated in terms of accuracy, linearity, method limits, reproducibility, and matrix effect. The proposed workflow combines sensitivity and robustness, with recoveries over 70%, method quantification limits less then 1 ng/L, and relative standard deviations less then 20% for most of the compounds. Slight matrix effect (ME) was ob

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