https://www.selleckchem.com/pr....oducts/folinic-acid.
Retrospective lineage tracing harnesses naturally occurring mutations in cells to elucidate single-cell development. Common single-cell phylogenetic fate mapping methods have utilized highly mutable microsatellite loci found within the human genome. Such methods were limited by the introduction of in vitro noise through polymerase slippage inherent in DNA amplification, which we characterized to be approximately 10-100× higher than in vivo replication mutation rate. Here, we present RETrace, a method for simultaneously capturing bo
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