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The effects of herbicide diuron on photosynthesis and vertical migration of intertidal microphytobenthos (MP assemblages were investigated using chlorophyll fluorometry. The results shown diuron ≤ 60 μg L-1 had no obvious effect on MPB vertical migration during 24 h indicated by consistent rhythm. Low concentration of 10 μg L-1 diuron had no significant influence on MPB photosynthesis throughout, however, high concentrations of 40, 50, and 60 μg L-1 had significant impacts exhibited by decreased parameters of maximum relative electron transport ra